General Cell Based Assays
Cayman Chemical 6-alkyn NAD sodIum salt 700ug
A clickable form of NAD+; has been used in conjunction with fluorescent tags in the detection of PARP protein substrates
Eurofins Discoverx PathHunter eXpress PTGFR U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0936E3CP5L)
The PathHunter PTGFR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. cloprostenol) based activation of PTGFR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGFR and EA-tagged β-Arrestin-2. Activation of PTGFR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter™ Human PTHR2 Gs/Gq Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human PTHR2 Gs/Gq Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure PTHR2 (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ILEX LIFE SCIENCES LLC Biocolor Fastin Elastin Assay, Standard Size Kit (110 assays)
The Biocolor Fastin™ Elastin Assay is a quantitative colorimetric dye-binding method for the analysis of elastins released into tissue culture medium and extracted from biological materials. The dye label employed is 5,10,15,20-tetraphenyl-21H,23H-porphine tetra-sulfonate (TPPS). Elastin forms that can be measured as α-elastin: soluble tropoelastins, lathyrogenic elastins, insoluble elastins (following solubilization to elastin polypeptides [α-elastin, κ-elastin]). In vitro application: soluble elastin secreted into cell culture medium may be measured directly. Samples should be free from particulate material. Tropoelastin is the elastin form usually found in cell-culture medium during in vitro cell culture. In vivo application: an extraction method of insoluble elastin from tissue and conversion to soluble α-elastin or soluble κ-elastin is given. After solubilization the samples are assayed by the procedure described for soluble elastin. Sensitivity: 5 µg. Assay Time: 4 hours.
Abcam Glutathione Peroxidase Assay Kit (Colorimetric)
Glutathione Peroxidase Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Enzo Life Sciences ALDetect (MDA-specific) Lipid Peroxidation assay kit (1x25tests)
Applications: Colorimetric detection. Contents: Reagent R1 (Prod. No. BML-KI527) (3x18ml;N-methyl-2-phenylindole, in acetonitrile). Reagent R2 (Prod. No. BML-KI606) (1x16.5ml; Conc. Hydrochloric acid). MDA Standard (Prod. No. BML-KI529) (1ml; 1,1,3,3-tetramethoxypropane (TMOP) in Tris-hydrochloric acid). BHT (Prod. No. BML-KI530) (2ml; Butylated hydroxytoluene in acetonitrile). Probucol (Prod. No. BML-KI531) (1.1ml; Probucol in methanol). Methanol (Prod. No. BML-KI532) (30ml)
Eurofins Discoverx AssayComplete Cell Culture Kit-112, (92-3112G)
AssayComplete Cell Culture Kit-112 is an optimized media kit intended for use in culturing and propagating Eurofins DiscoverX cell lines. This kit contains the base medium, relevant serum, and pen/strep required to produce a full cell culture medium for optimal cell culture results. This product is provided as a 3-component kit which will produce approximately 550 mL of finished media. Please refer to cell line specific datasheet to determine the recommended cell culture kit. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx cAMP Hunter™ Human CHRM2 Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human CHRM2 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CHRM2 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Biolegend Alexa FluorR 594 anti-beta-act
Alexa Fluor 594 anti-β-actin [2F1-1]; Isotype: Mouse IgG2b, κ; Reactivity: Human, Mouse, Rat; Apps: ICC, IHC-P; Size: 100 μg
Revvity Health Sciences Inc HTRF mAb Anti-6His Eu-Conjugate, 5,000 Assay Points
HTRF mAb Anti-6His Eu-Conjugate, 5,000 Assay Points
Cell Signaling Technology PathScanR Phospho-AMPK-alpha Thr172 Chemiluminescent Sandwich ELISA Kit 10 Kit
PathScanR Phospho-AMPK-alpha Thr172 Chemiluminescent Sandwich ELISA Kit 10 Kit
AnaSpec SensoLyte Red Glucocerebrosidase (GBA) Assay Kit *Fluorimetric*
The SensoLyte Red Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a highly sensitive fluorogenic substrate. The GBA substrate provided in the kit releases the red fluorescent dye resorufin upon Glucocerebrosidase cleavage with absorption/emission maxima at 570 nm/610 nm. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. The Red Glucocerebrosidase (GBA) Assay is one-step homogenous reaction, which does not require the additional step of dispensing stop solution after the incubation, thereby suitable for HTS. The long wavelength fluorescence resorufin is also less interfered by the autofluorescence of components in biological samples and test compounds.
Cayman Chemical GlycolysIs Cel-Based asy K 1ea
Cayman’s Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase (LDH) catalyzes the reaction between NAD+ and lactate, yielding pyruvate and NADH. The NADH directly reduces a tetrazolium salt (INT) to a colored formazan which absorbs between 490 and 520 nm. The quantity of formazan produced is proportional to the quantity of lactate in the culture medium and is thus an indirect measurement of glycolysis. This assay can be adapted to high-throughput screening to examine glycolytic regulators in a cell-based system. In addition, the non-invasive sampling leaves the cells intact and thus allows multiplex testing for additional markers.
Eurofins Discoverx PathHunter eXpress IL5RA/CSF2RB Dimerization Assay, 10pl Kit. (93-0972E3CP0L)
The PathHunter IL5RA/CSF2RB Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-5) induced dimerization of IL5RA/CSF2RB receptors. This product uses EFC technology. The cell line included overexpresses CSF2RB target protein fused to PK alongside IL5RA target protein fused to EA. Upon ligand-induced dimerization of IL5RA/CSF2RB, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress AVPR1A U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0587E3CP0L)
The PathHunter AVPR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. vasopressin) based activation of AVPR1A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AVPR1A and EA-tagged β-Arrestin-2. Activation of AVPR1A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.